StayBrite™ Highly Stable ATP Bioluminescence Assay Kit

Adenosine-5’-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and disease stages, etc.. An accurate and reliable detection of ATP level has wide applications. Luciferase/Luciferin has been used to sensitively measure ATP levels. Conventional Luciferase/Luciferin ATP detection systems, however, are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5x10ᶦᶦRLU/ mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.