Alcohol Dehydrogenase Activity Colorimetric Assay Kit

Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. BioVision’s Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the ADH in a variety of samples. In the assay ADH utilizes ethanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (λ = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.