PicoProbe™ Hexokinase Activity Assay Kit (Fluorometric)

Hexokinase (EC 1.1.1.49; also called HK, 6-Phosphate Glucose Kinase, ATP: D-Hexose 6-Phosphotransferase, ATP-dependent Hexokinase) is found in many organisms including bacteria, plants and mammals. Hexokinases play an important role in glucose metabolism. There are four isoforms (HK-I, II, III and IV). HK-I, HK-II, and HK-III have low Km, while HK-IV (also called Glucokinase) has 100-fold high Km for glucose. Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. BioVision’s PicoProbe™ Hexokinase Activity Assay kit provides a quick and easy method for monitoring HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-Phosphate, which in turn undergoes a series of reactions and reduce PicoProbe™ to generate intense fluorescent product (Ex/Em = 535/587 nm). The assay is simple, sensitive and high-throughput adaptable and can detect as low as 2 µU of HK activity.