PAF Acetylhydrolase Activity Assay Kit (Colorimetric)

Platelet-Activating Factor (PAF or 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is an important phospholipid mediator, which has diverse biological activities. PAF is synthesized and secreted by a variety of cells such as mast cells, monocytes, macrophages etc. Upregulated PAF signaling can cause pathological inflammation and also has been found to be responsible for sepsis, shock, and traumatic injury. PAF Acetylhydrolase (PAF-AH or 1-alkyl-2-acetylglycerophosphocholine esterase or Lipoprotein-associated phospholipase A2 or Lp-PLA2) (EC 3.1.1.47) hydrolyzes PAF by removing acetyl group at the sn-2 position and converts PAF into biologically inactive form, lyso-PAF. PAF-AH has two forms: extracellular and intracellular that shares some similarities. In human, PAF-AH deficiency leads to severe asthma. Therefore early detection of PAF-AH activity is critical for mechanistic study, diagnosis, prevention, and therapeutic purpose. BioVision’s PAF Acetylhydrolase Assay kit provides a quick and easy way for monitoring PAF-AH activity in a variety of samples. In this kit, PAF-AH converts PAF into an inactivated form and probe gets converted into a colored product with strong absorbance at 412 nm. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU.