Cortisol (Saliva) Human ELISA Kit

BioVision’s Cortisol (Saliva) Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of Cortisol in saliva. Cortisol is a steroid hormone released from the adrenal cortex in response to the hormone ACTH. It increases blood pressure and blood sugar levels, and suppresses the immune system. Cortisol acts through specific intracellular receptors and has effects on numerous physiologic systems, including immune system, glucose-counter regulation, vascular tone, substrate utilization, and bone metabolism. It exists in the blood as either a free form or bound to corticosteroid-binding globulin (CBG). While only 1-10% of Cortisol in the blood exists in the unbound/free form, this fraction represents the active pool of Cortisol eliciting the biological response, and is capable of diffusing into target tissues and saliva. Studies consistently report high correlation between salivary and serum cortisol levels, indicating that salivary cortisol can reliably estimate serum Cortisol levels. BioVision’s Cortisol (Saliva) ELISA kit is a competitive enzyme immunoassay kit. The assay employs an antibody specific for cortisol coated on a 96-well plate. Cortisol in a sample and HRP labeled Cortisol compete for binding to the immobilized antibody. After simultaneous incubation, the excess reagents are washed away and a TMB substrate is added to the wells. Color develops in proportion to the amount of bound Cortisol HRP conjugate, and the intensity is inversely proportional to the concentration of Cortisol in the sample. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 1 ng/ml and detection range is from 1 ng/ml to 100 ng/ml. The cross-reactivity of this ELISA assay is 6.8% for prednisolone, 4.22% for Cortisone, and <0.1% for other hormones, including 11-deoxycortisol, estradiol, testosterone, and progesterone.