Triiodothyronine (T3) (human) ELISA Kit

Triiodothyronine (T3) is a hormone produced by the thyroid gland. T3 plays an important role in a majority of the physiological processes in the body such as growth, development, metabolism, heart rate, and body temperature. The hormone exists in 2 forms: almost all of T3 is mainly bound to plasma proteins namely Thyroxine-binding globulin and serum albumin. The free unbound T3 is the biologically active form of the hormone. The anterior pituitary gland produces the Thyroid-stimulating hormone which further stimulates the production of T3, along with thyroxine (T4). The 3 hormones are engaged in a closed-loop feedback process; as the concentrations of T3 and T4 increase in the blood plasma, the anterior pituitary stops the production of TSH; when T3 and T4 concentrations decrease, TSH production increases. If an excess amount of T3 and T4 is produced by the thyroid gland, it results in hyperthyroidism whereas insufficient amounts lead to hypothyroidism. BioVision’s Triiodothyronine (T3) (Human) ELISA Kit is designed to measure the amount of total Triiodothyronine (bound as well as unbound forms of T3) in human serum samples and to evaluate the functioning of the thyroid gland. The kit is based on the Competitive ELISA principle. Samples, Standards, Assay diluent, and T3 enzyme conjugate are added to the wells coated with an anti-T3 monoclonal antibody. The T3 present in the samples will compete with the T3 enzyme conjugate for binding to the antibody. Any unbound T3 from the serum and enzyme conjugate is washed off with the wash buffer. The addition of the substrate results in color development that is inversely correlated to the concentration of the T3 in the samples.