PicoProbe™ Reduced Glutathione (GSH) Assay Kit (Fluorometric)

Glutathione (GSH), a thiol-containing tripeptide, is a key antioxidant in living systems. Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell. BioVision’s PicoProbeTM Reduced Glutathione Assay Kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). Our assay is the most specific and sensitive in the market. Oxidized Glutathione does not interfere with the assay. The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction