Lactate Dehydrogenase Activity Colorimetric Assay Kit

Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) present in a wide variety of organisms. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NADᶧ. When disease or injury or toxic material damages tissues, cells release LDH into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. In this colorimetric LDH quantification assay, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive. The kit can detect 1 -100 mU/ml of LDH directly in samples.