PicoProbe™ Lactate Fluorometric Assay Kit

Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP. BioVision’s PicoProbe™ Lactate Assay kit is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid & high-throughput suitable assay kit is the most sensitive Lactate Assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.