Sucrose Colorimetric/Fluorometric Assay Kit

Sucrose (C₁₂H₂₂O₁₁; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. BioVision’s Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.