D-Amino Acid Oxidase Activity Assay Kit (Fluorometric)

D-amino acid oxidase (DAAO, DAO, EC 1.4.3.3) is a flavoprotein which oxidizes a D-amino acid forming hydrogen peroxide and the respective imino acid which in a second catalytic step by he same enzyme is hydrolyzed to a α-keto acid and ammonia. In recent years, DAAO has been studied for its possible role in schizophrenia. The simultaneous expression of DAAO and a putative product from G72 gene may potentially increase the risk of schizophrenia in patients. For example, increased DAAO expression and enzymatic activity have been reported in post mortem brain tissue samples from patients with schizophrenia compared to healthy controls. DAAO expression in human brain is very specific: it can only be found in the cerebellum and is not detectable in any other parts of the brain. DAAO regulates the physiological levels of D-amino acids such as D-serine, D-aspartate and D-alanine. D-serine acts as a modulator of N-Methyl-D-aspartate receptor (NMDA receptor, NMDAR), while D-aspartate and D-alanine are found to be elevated in both white matter and gray matter in patients’ brains suffering from Alzheimer disease. BioVision’s D-amino Acid Oxidase Activity Assay Kit provides a quick and easy method for monitoring DAAO activities in a wide variety of samples. In this assay, DAAO converts D-amino acid into hydrogen peroxide as one byproduct. Consequently, the formed byproduct will react with a probe producing a strong fluorescent signal (Ex/Em= 535/587nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.5 µU of DAAO activity