PicoProbeTM Tyrosine Aminotransferase Activity Assay Kit (Fluorometric)

Tyrosine Aminotransferase (EC 2.6.1.5), also known as Tyrosine Transaminase (TAT), is a mitochondrial enzyme encoded by the TAT gene in the nuclear DNA. It catalyzes the transamination of tyrosine by transferring an amine group to an α-ketoglutarate molecule. Tyrosine Aminotransferase is highly expressed in the liver and its deficiency, usually inherited, leads to a buildup of toxic levels of tyrosine in the body called tyrosinemia. Tyrosinemia causes skin and corneal lesions, kidney and liver disorders, and could lead to mental retardation in newborns. BioVision’s PicoProbeTM Tyrosine Aminotransferase Activity Assay Kit is a simple plate-based fluorometric assay for the measurement of TAT activity in biological samples. The transamination of tyrosine by TAT produces glutamate (through the transfer of amine group to α-ketoglutarate), which converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 4 µU of tyrosine aminotransferase.