p53 (human) ELISA Kit

BioVision’s p53 Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of native and recombinant human p53. The p53 protein is encoded by the TP53 gene. It is composed of 393 amino acids but its apparent molecular mass is 53 kDa. p53 binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. It acts as a pivotal suppressor of inappropriate cell proliferation. By initiating suppressive effects through induction of apoptosis, cell senescence, or transient cell-cycle arrest, p53 plays an important role in cancer suppression, developmental regulation, and aging. p53 is found in increased amounts in a wide variety of transformed and tumor cells, where its concentration is increased 5-1000 fold over the concentration in normal cells. Meanwhile, p53 is also frequently mutated or inactivated in about 60% of cancers. The p53 ELISA assay employs an antibody specific for human p53 coated on a 96-well plate. Standards and samples are pipetted into wells and p53 present in the sample is bound to the wells by immobilized antibody. Wells are washed and a biotin-labeled human p53 specific detection antibody is added. After washing away unbound detection antibody, a streptavidin HRP-conjugate is added to the wells. The wells are again washed, a TMB substrate solution is added, and color develops in proportion to the amount of bound p53. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 0.1 ng/ml and detection range is from 0.2 ng/ml to 25.6 ng/ml. Recovery within this range is 86.3%. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.