Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)

Soluble Epoxide Hydrolase or sEH (EC 3.3.2.10) is a cytosolic enzyme present ubiquitously in several organs including the liver, kidney, pancreatic islets, pituitary gland, lymphoid tissues, muscles, and the gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), i.e., epoxides derived from cytochrome P-450 mediated metabolism of arachidonic acid, and forming vicinal diols. sEH plays a major role in metabolism of endogenous lipids that are implicated in pain and inflammation. BioVision’s Soluble Epoxide Hydrolase Activity Assay Kit is a microplate based fluorometric kit for measuring sEH activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activitys.