EZClick™ Global Protein Synthesis Assay Kit

Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc). It is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical in determining the physiological state of the cell. Methods of detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important for assessment of cytotoxicity. Biovision’s EZClick™ Global Protein Synthesis Assay Kit utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em= 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in the real-time, and Cycloheximide, an inhibitor of protein synthesis that serves as a control