Total D-Amino Acid Assay Kit (Fluorometric)

D-Amino acids are present in both eukaryotes and prokaryotes. D-Amino acids act as a component of the cell wall in prokaryotes and a neurological regulator in eukaryotes. In mammals, D-Amino acids play an important role in physiological functions and their levels are regulated by D-Amino acid oxidase in the central nervous systems. Some important D-Amino acids include D-serine, D-aspartate and D-alanine. D-serine acts as a modulator of N-Methyl-D-aspartate receptor (NMDA receptor, NMDAR), while D-aspartate and D-alanine have been found to be elevated in the white and the gray matter respectively in patients’ brains suffering Alzheimer’s disease. The abnormal levels of D-Amino acids could be used as a marker of neurological diseases: total D-Amino acid concentration in cerebrospinal fluid (CSF) has been shown to be higher in patients with Alzheimer’s disease when compared to samples from healthy donors. BioVision’s Total D-Amino Acid Assay Kit (Fluorometric) provides a quick, specific and easy method for measuring total D-Amino acid concentrations in a wide variety of samples. In this assay, D-Amino acids are converted into an intermediate by the DAA enzyme mix that will further react with a probe to produce a strong fluorescence signal (Ex/Em= 535/587nm). The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1.9 μM of D-Amino acids in biological samples.