QuickDetect™ VEGF (Human) ELISA Kit

Vascular endothelial growth factor (VEGF) is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. BioVision’s QuickDetect™ VEGF ELISA kit is a sandwich ELISA assay for measuring the quantity of human VEGF in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.

Product Specifications

CAS Number

7732-18-5

Reactivity

Human

Applications

This ELISA kit is used for quantitative measurement of VEGF in human serum, plasma, cell culture supernatant and other biological fluids.

Detection Method

Absorbance (450 nm)

Features & Benefits

Easy, convenient and time-saving method to measure the level of VEGF in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results. Detection Range: 5 - 350 pg/ml Sensitivity: < 0.8 pg/ml Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100) Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.