QuickDetect™ Free-PSA (Human) ELISA Kit

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme and a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. Most PSA in the blood is bound to serum proteins. A small amount is not protein bound and is called 'free PSA'. Measuring the ratio of free to total PSA appears to be particularly promising for eliminating unnecessary biopsies in men with PSA levels between 4 and 10 ng/mL. BioVision’s QuickDetect™ free-PSA ELISA kit is a sandwich ELISA assay for measuring the quantity of human free-PSA in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash step respectively, resulting in less errors and more consistent results.

Product Specifications

CAS Number

7732-18-5

Reactivity

Human

Applications

This ELISA kit is used for quantitative measurement of free-PSA in human serum, plasma, cell culture supernatant and other biological fluids.

Detection Method

Absorbance (450 nm)

Features & Benefits

Easy, convenient and time-saving method to measure the level of free-PSA in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results. Detection Range: 3 - 200 pg/ml Sensitivity: < 0.5 pg/ml Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100) Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.