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HDAC3 Inhibitor Screening Kit (Fluorometric)

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.

Product Specifications

Features & Benefits

• Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for testing putative HDAC3 inhibitors

Shipping Conditions

gel pack

Storage Conditions

-20°C

Shelf Life

12 months

Frequently Asked Questions

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