Caspase-4 Colorimetric Assay Kit
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Product Specifications
Features & Benefits
• Simple one-step procedure; takes only 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity.
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
6-12 months
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