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Caspase-4 Colorimetric Assay Kit

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Product Specifications

Features & Benefits

• Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent Caspase activity.

Shipping Conditions

gel pack

Storage Conditions

-20°C

Shelf Life

6-12 months

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