ExoDNAPS™ Circulating and Exosome-associated DNA Extraction Kit (Human Plasma/Serum, 20 reactions)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. Circulating DNA is emerging as a novel non-invasive tool for patient’s stratification and disease monitoring. While most of the research has focused on circulating cell-free (cfDNA) or circulating-tumor-cell (CTC) derived DNA, extracellular vesicle (EVs) associated DNA (EV-DNA) is emerging as a third valuable “liquid biopsy” platform. Genomic single or double-stranded DNA and mitochondrial DNA have been recently detected in exosomes and microvesicles. In particular, the majority of the double-stranded DNA seems to be associated with tumor derived exosomes and can be an important new source of biomarkers for tumor detection. ExoDNAPS™ Kit (isolation and purification of circulating and Exosome-associated DNA) combines the ability of our ExoPure™ reagent to isolate EVs and circulating DNA from biofluids (plasma and serum) with a user friendly system of DNA purification. Isolated vesicles are lysed with the appropriate lysis buffer and DNA is purified by and exosome DNA is purified by spin columns and optimized buffers with a fast turnaround time (~30 min). The kit provides an appropriated DNA concentrator for concentrating the yield (4-fold concentration) and increasing the purity of the DNA to the levels required for digital PCR analysis.