TNF alpha (rat) ELISA Kit

BioVision’s rat TNF alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for rat TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TNF alpha captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (rat serum, plasma). Sensitivity: < 1 pg/ml.