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Recombinant Human Desert hedgehog protein (DHH) (Active)

Product Specifications

Background

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Product Name Alternative

DHH

Gene ID

50846

UniProt

O43323

Target

IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

Purification

>96% as determined by SDS-PAGE and HPLC.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1,2 (CCL-226) cells is 15-45 g, ml.

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Calculated Molecular Weight

19.9 kDa

Formulation

0.2 ?m filtered 20 mM PB, pH 7.4, 150mM NaCl , lyophilized

Host or Source

E.Coli

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