Biotinylated Chimeric HLA-A*02:01 (H-2Kb α3) &B2M&GP100 (YLEPGPVTA) Complex Protein (Monomer, MALS verified)

Glycoprotein 100, is also well known as gp100 and Melanocyte protein PMEL. This antigen is a melanocyte-specific type I transmembrane glycoprotein and composed of 661 amino acids. Glycoprotein 100 is related to the tumor regression in metastatic melanoma patients after adoptive therapy, by which its epitopes being recognized by tumor lymphocytes and T-cells. The gp100280–288 (YLEPGPVTA) was originally shown to be recognized by HLA-A*0201 tumor-infiltrating lymphocytes from melanoma patients, and therefore it is widely been studied in TCR -T studies. The Human HLA-A*0201 Mouse H-2Kb gp100 (YLEPGPVTA) complex protein is a complex of HLA-A*0201 & H-2Kb of the MHC Class I, hB2M and YLEPGPVTA peptide of the gp100.

Product Specifications

Background

Specifications

Biotinylated Chimeric HLA-A*02:01 (H-2Kb α3) &B2M&GP100 (YLEPGPVTA) Complex Protein is produced by co-expression of HLA, H-2Kb and B2M loaded with GP100 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 50.3 kDa. The protein migrates as 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

YLEPGPVTA & P61769 (B2M) & AAA59606.1 (HLA-A*02:01) & P01901 (H-2Kb)

Expression Region

YLEPGPVTA peptide & Ile 21 - Met 119 (Human B2M) & Gly 25 - Thr 206 (Human HLA-A*02:01) & Asp 204 - Trp 295 (Mouse H-2Kb)

Host

HEK293

Target

Chimeric HLA-A*0201 (H-2Kb α3) & B2M & GP100 (YLEPGPVTA)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human & Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.