What is the catalog number for EDF1/MBF1 Antibody?

The catalog number for EDF1/MBF1 Antibody is orb570253.

What sizes are available for EDF1/MBF1 Antibody?

EDF1/MBF1 Antibody is available in the following size(s): 100 µg.

Is EDF1/MBF1 Antibody in stock?

EDF1/MBF1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

EDF1/MBF1 Antibody

Goat polyclonal antibody to EDF1

Product Specifications

Product Name Alternative

EDF1; MBF1; EDF-1; MGC9058; endothelial differentiation-related factor 1; multiprotein bridging factor 1

Reactivity

Human, Mouse

Target

EDF1 / MBF1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

AESDWDTVTVLRK

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

16.4; 15.5; 15.6; 14.1; 14.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 19kDa band observed in nuckear lysates of cell line HEK293, approx. 18kDa in nuckear lysates of cell line HeLa, and approx 17kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4kDa according to Human NP_003783.1 and Mouse NP_067494.1) .. IHC: Paraffin embedded Human Pancreas and Brain (Cortex) . Recommended concentration: 3.75μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Prediction Reactivity

Rat

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.