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CD74 (aa159-171) Antibody

Goat polyclonal antibody to CD74

Product Specifications

Product Name Alternative

Anti CD74 antibody, anti DHLAG antibody, anti HLADG antibody, anti Ia-GAMMA antibody, anti CD74 molecule, major histocompatibility complex, class II invariant chain antibody, anti CD74 antigen antibody, anti CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody, anti HLA-DR-gamma antibody, anti Ia-associated invariant chain antibody, anti MHC HLA-DR gamma chain antibody, anti gamma chain of class II antigens antibody

Reactivity

Human

Target

CD74 (aa159-171)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KGSFPENLRHLKN

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: This antibody was observed to recognize variations of bands at approx. 26kDa, 30kDa,35kDa and 70kDa in Human Duodenum and Lung lysates (calculated MW of 26.4kDa according to NP_004346.1 and 33.5kDa according to NP_001020330.1. All bands were blocked by incubation with the immunizing peptide and have been observed by other commercial sources. Recommended concentration: 0.5-1μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5μg/ml Flow Cytometry: Flow cytometric analysis of Caco--2 cells. Recommended concentration: 10ug/ml.

Molecular Weight

33.5; 26.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, FC, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

972

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