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Fgf14 (mouse N terminus) Antibody

Goat polyclonal antibody to Fgf14

Product Specifications

Product Name Alternative

Anti Fgf14 antibody, anti FGF-14 antibody, anti Fhf4 antibody, anti FHF-4 antibody, anti fibroblast growth factor 14 antibody, anti fibroblast growth factor homologous factor 4 antibody, anti mFHF-4 (1B) antibody

Reactivity

Human, Mouse

Target

Fgf14 (mouse N terminus)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

REQHWDRPSASR

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 1:5000

Molecular Weight

27.7; 28.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2) . Recommended concentration: 1-3μg/ml. Primary incubation was 1 hour.. IHC: Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.

Prediction Reactivity

Bovine, Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2259

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