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CPLA2-zeta Antibody

Goat polyclonal to CPLA2-zeta

Product Specifications

Product Name Alternative

Anti PLA2G4F antibody, anti phospholipase A2, group IVF antibody, anti DKFZp666G192 antibody, anti DKFZp781B229 antibody, anti PLA2G4FZ antibody, anti cytosolic phospholipase A2 zeta antibody, anti cPLA2-zeta antibody

Target

CPLA2-zeta

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QTAEEKAFGDFVINR

Field of Research

Cardiovascular Disease

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1 μg/ml

Molecular Weight

95.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Human, Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

255189

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