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DTX3 Antibody

Goat polyclonal antibody to DTX3

Product Specifications

Product Name Alternative

Anti DTX3 antibody, anti deltex 3 homolog (Drosophila) antibody, anti FLJ34766 antibody, anti MGC138863 antibody, anti MGC138864 antibody, anti RNF154 antibody, anti deltex 3 homolog antibody

Reactivity

Human, Mouse

Target

DTX3

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KNKVTVSKPVWD

Field of Research

Stem Cells

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.2-0.5 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

38

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1) . The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278 (24) :21930-7.; PMID: 12670957) . Recommended concentration: 0.2-0.5 μg/ml.

Prediction Reactivity

Bovine, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

196403

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