SRF Antibody
Goat polyclonal antibody to SRF
Product Specifications
Product Name Alternative
SRF; serum response factor (c-fos serum response element-binding transcription factor) ; MCM1; OTTHUMP00000039820
Reactivity
Human
Target
SRF
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QASPSRDSSTDLTQ
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml
Molecular Weight
51.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol. 2002 Apr;282 (4) :H1521-33.; PMID: 11893590) . Recommended concentration: 1-3 μg/ml.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6722
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