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Goat anti-HTR7 / 5-HT7 Antibody

Goat polyclonal antibody to HTR7

Product Specifications

Product Name Alternative

Anti HTR7 antibody, anti 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) antibody, anti 5-HT7 antibody, anti 5-hydroxytryptamine receptor 7 antibody, anti OTTHUMP00000020077 antibody, anti OTTHUMP00000020078 antibody, anti OTTHUMP00000020079 antibody, anti serotonin 5-HT-7 receptor antibody

Reactivity

Human

Target

HTR7 / 5-HT7

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QNADYCRKKGHDS

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml

Molecular Weight

49.5; 48.0; 53.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in lysates of cell line KELLY (calculated MW of 49.5kDa according to NP_000863.1) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

3363

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