AIRE (Human) ELISA Kit

Autoimmune regulator (AIRE) is a key transcription factor that is expressed in the epithelial cells of the thymic medulla (mTECs). Besides the thymus, AIRE is also expressed in peripheral lymphoid organs and tissues such as the spleen, lymph nodes, embryonic liver, testis, and ovaries. However, its role in peripheral tolerance is less known. AIRE regulates immune tolerance by clearing auto-reactive T cells and induce the production of Treg cells. AIRE mutations result in a rare autoimmune disorder called autoimmune polyglandular syndrome type 1 (APS-1), also known as autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). BioVision’s AIRE (Human) ELISA kit is to quantitatively measure the amount of AIRE in human serum, plasma, and other biological fluids. The kit is based on the Sandwich ELISA principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of AIRE in the sample or standard.