USP28 Antibody
Goat polyclonal antibody to USP28
Product Specifications
Product Name Alternative
Anti USP28 antibody, anti ubiquitin specific peptidase 28 antibody, anti KIAA1515 antibody, anti ubiquitin specific protease 28 antibody
Reactivity
Human
Target
USP28
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SVELKHYIQEDN
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.2 μg/ml, IHC-P: 2.5-3.8μg/ml
Molecular Weight
122
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 122kDa according to NP_065937.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
57646
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