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Goat anti-APOL4 (C Terminus) Antibody

Goat polyclonal antibody to APOL4

Product Specifications

Product Name Alternative

Anti apolipoprotein L4 antibody, anti APOL4 antibody, anti apolipoprotein L, 4 antibody, anti APOL-IV antibody, anti APOLIV antibody, anti OTTHUMP00000028937 antibody, anti apolipoprotein L-IV antibody

Reactivity

Human

Target

APOL4 (C Terminus)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ENLNELTHIHQSLK

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

38.7; 39.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2) . Recommended concentration: 0.3-1 μg/ml. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported We call for caution when the antibody is used for other assays than Western blot.

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

80832

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