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Recombinant NKX2.2 Antibody / Rabbit Monoclonal

NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity) . [UniProt]

Product Specifications

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Clonality

Recombinant

Clone

RMNK2-1

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Protein A affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Rabbit

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Rabbit IgG, kappa

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