MARCH-VII/Axotrophin Antibody
Goat polyclonal antibody to 41705
Product Specifications
Product Name Alternative
Anti MARCH7 antibody, anti membrane-associated ring finger (C3HC4) 7 antibody, anti AXO antibody, anti AXOT antibody, anti DKFZP586F1122 antibody, anti MARCH-VII antibody, anti RNF177 antibody, anti axotrophin antibody, anti membrane-associated RING-CH protein VII antibody
Reactivity
Human
Target
MARCH-VII / Axotrophin
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NFNQESEGRNT
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml
Molecular Weight
78.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 125kDa band in Human Spleen and Tonsil lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_073737.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
64844
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