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B2M Antibody

Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC) . Involved in the presentation of peptide antigens to the immune system. [UniProt]

Product Specifications

UniProt

P61769

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the B2M antibody.

Clonality

Monoclonal

Clone

MGBP2-1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 2-4ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the B2M antibody may be required for optimal performance.>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2b, kappa

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