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MUC3 Antibody

The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats. Mucin-3/MUC3 is a major glycoprotein component of a variety of mucus gels. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. May be involved in ligand binding and intracellular signaling. [UniProt]

Product Specifications

UniProt

Q02505

Reactivity

Human

Immunogen

Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.

Clonality

Monoclonal

Clone

MUCN3-1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the MUC3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

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