What is the catalog number for TOP1MT Antibody?

The catalog number for TOP1MT Antibody is orb2641625.

What sizes are available for TOP1MT Antibody?

TOP1MT Antibody is available in the following size(s): 100 µg.

Is TOP1MT Antibody in stock?

TOP1MT Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

TOP1MT Antibody

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Product Specifications

UniProt

Q969P6

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.

Clonality

Monoclonal

Clone

TPIMT-1

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the TOP1MT antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.