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Recombinant Perilipin 2 Antibody / PLIN2 / Adipophilin / ADRP

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [RefSeq]

Product Specifications

UniProt

Q99541

Reactivity

Human

Immunogen

A portion of amin acids 249-376 from the human protein were used as the immunogen for this recombinant Perilipin 2 antibody.

Clonality

Recombinant

Clone

ADPN1-2R

Conjugation

Unconjugated

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml, Prediluted IHC only format: incubate for 30 min at RT (1), Western blot: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the recombinant Perilipin 2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P, WB

Host or Source

Rabbit

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rabbit IgG, kappa

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