Ki67 Antibody
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]
Product Specifications
UniProt
P46013
Reactivity
Human
Immunogen
A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.
Clonality
Monoclonal
Clone
PMKI67-1
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa
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