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Recombinant Calponin Antibody

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Product Specifications

UniProt

P51911

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.

Clonality

Recombinant

Clone

RCNN1/832

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Dilution

Western blot: 0.5-1ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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