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Recombinant Cytokeratin 16 Antibody / Rabbit Monoclonal

Cytokeratin 16 is an epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. [UniProt]

Product Specifications

UniProt

P08779

Reactivity

Human

Immunogen

A C-terminal region peptide was used as the immunogen for the recombinant Cytokeratin 16 antibody.

Clonality

Recombinant

Clone

CTKN16-1R

Conjugation

Unconjugated

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format : incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Cytokeratin 16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Rabbit

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rabbit IgG, kappa

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