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Recombinant Cytokeratin 7 Antibody

CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7) . Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]

Product Specifications

UniProt

P08729

Reactivity

Human

Immunogen

OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the recombinant Cytokeratin 7 antibody.

Clonality

Recombinant

Clone

ROV-TL12/30

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting amounts. Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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