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Recombinant E-Cadherin Antibody / Rabbit Monoclonal

Cadherins behave as both receptors and ligands for other molecules. During development, their behavior assists in properly positioning cells: they are responsible for the separation of the different tissue layers, and for cellular migration. In the very early stages of development, E-cadherin (epithelial cadherin) is most greatly expressed. The E-cadherin-catenin complex plays a key role in cellular adhesion; loss of this function has been associated with greater tumour metastasis. [Wiki]

Product Specifications

UniProt

P12830

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the recombinant E-Cadherin antibody.

Clonality

Recombinant

Clone

ECD1-3R

Conjugation

Unconjugated

Purification

Protein A affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunofluorescence: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format : incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Rabbit

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rabbit IgG, kappa

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