What is the catalog number for Recombinant Bcl-2 Antibody?

The catalog number for Recombinant Bcl-2 Antibody is orb2640493.

What sizes are available for Recombinant Bcl-2 Antibody?

Recombinant Bcl-2 Antibody is available in the following size(s): 100 µg.

Is Recombinant Bcl-2 Antibody in stock?

Recombinant Bcl-2 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant Bcl-2 Antibody

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative.

Product Specifications

UniProt

P10415

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the recombinant Bcl-2 antibody.

Clonality

Recombinant

Clone

RBCL2/796

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.