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S100A1 Antibody

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain, but not on the beta-chain, of S100. This antibody can be used to localize S-100A in various tissue sections. S100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells.

Product Specifications

UniProt

P23297

Reactivity

Human

Immunogen

Human recombinant S100A1 full length protein was used as the immunogen for this S100A1 antibody.

Clonality

Monoclonal

Clone

S100A1/1942

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE-not suitable for frozen tissues) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the S100A1 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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