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CDw78 Antibody

This mAb reacts with a carbohydrate determinant, identified as CDw78 (Workshop V; Code CDw78.2) . The epitope recognized by this mAb and Leu21 is the same. It is present on some immature and some peripheral blood B cells, and some B cell lines. Its expression is increased after B-cell activation in vitro. CDw78 is also found on tissue macrophages and on epithelial cells, but not on T cells, NK cells, monocytes, granulocytes and myeloid tissues. It appears that CDw78 is a determinant on a major histocompatibility complex (MHC) class II subpopulation.

Product Specifications

Reactivity

Human

Immunogen

Human leukocytes were used as the immunogen for the CDw78 antibody.

Clonality

Monoclonal

Clone

DF1588

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CDw78 antibody should be determined by the researcher.

Tested Applications

FACS, Functional Studies, IF

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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