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CFTR Antibody

Cystic fibrosis transmembrane conductance regulator (CFTR) is involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. [UniProt]

Product Specifications

UniProt

P13569

Reactivity

Human

Immunogen

A partial recombinant protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this CFTR antibody.

Clonality

Monoclonal

Clone

CFTR/1643

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

Tested Applications

FACS, IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2b, kappa

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